Identification and developmental expression of the full complement of cytochrome p450 genes in zebrafish. Zebrafish were the first glofish available in pet stores, and are now sold in bright red, green, orangeyellow, blue, pink, and purple fluorescent colors. The use of zebrafish danio rerio as a model organism began in the 1960s. Danio rerio commonly known as the zebrafish is a tropical fish belonging to the minnow family cyprinidae, commonly kept in aquaria and used for scientific research. We appreciate as much detail as possible and references as appropriate. Effects of copper oxide nanoparticles on developing zebrafish embryos and larvae yan sun, gong zhang, zizi he, yajie wang, jianlin cui, yuhao li department of pathology, key laboratory of tumor microenvironment and neurovascular regulation, nankai university school of medicine, tianjin, peoples republic of china abstract. Product description type unit quantity academic price book. The zebra barred dartfish is also referred to as the zebra dart goby, bar goby, barred dartfish, zebra goby, or chinese zebra goby.
Zebrafish danio rerio has been a prominent model vertebrate in a variety of biological disciplines. The expression of cyp1a mrna in zebrafish exposed to 0. Substantial information gathered from developmental and genetic research, together with nearcompletion of the zebrafish genome project, has placed zebrafish in an attractive position for use as a toxicological model. Screening injected fish for developmental phenotypes, such as changes in the assembly of blood vessels, provides insight into the genetic control of complex developmental processes. The tiny fish thats changing modern medicine youtube. C leflunomide docking orientation into ahr2, d ahr1b and e ahr1a homology model binding pockets. Cyp1a reporter zebrafish reveals target tissues for dioxin. Isolation and functional characterization of a dioxin. The zebrafish danio rerio has been extensively used in biomedical research as a model to study vertebrate development and hematopoiesis and recently, it has been adopted into varied fields including immunology. Functional differences in the cytochrome p450 1 family. A variety of different glofish are currently on the market. Generation and application of a novel transgenic zebrafish.
Some species are more commonly known by the names lionfish and turkeyfish this article was most recently revised and updated by amy tikkanen, corrections manager. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. Zebrafish, danio rerio, are a species of freshwater, tropical fish within the minnow family i. Chapter 9 molecular methods preparation of genomic dna source. A functioning cyp1 ahr2 feedback loop is crucial for regulation of ahr2 signaling by a potential physiological ligand in vivo. The zebrafish, however, offers considerably more as a model species and is now used in many different research fields including basic developmental biology, neurobiology, and immunology. Zebrafish as a model vertebrate for investigating chemical. Xenobiotic metabolism and zebrafish danio rerio larvae by huw simon jones a thesis submitted to the university of birmingham. To support observations on changes in cyp1a expression, evaluation of cyp1a enzymatic activity was assessed in parallel by evaluation of. Integrates and synthesizes the biology of the zebrafish under one coverfeatures contributions from the leading researchers in their fieldsreaches a wider audience of researchers and. The zacs wt fish have been purchased from zirc, sarl, and boston childrens hospital we. Isolation and functional characterization of a dioxininducible cyp1a regulatory region from zebrafish danio rerio. The zebrafish is a shoaling fish and it should never be kept in groups of less than 68 fish. Previous research provided information on adverse effects of sulfolane on mammals, but relatively little information is available on.
A tcdd docking orientation in zebrafish ahr2 and b ahr1blbd homology model binding pocket icm v3. To this end, zebrafish embryos were exposed to the weak agonists carbaryl carb, phenanthrene phe, 2methylindole 2mi, 3methylindole 3mi, indigo, and indirubin individually, and cyp1a activity and cardiac toxicity were measured. Increasing use of zebrafish in drug discovery and mechanistic toxicology demands knowledge of cytochrome p450 cyp gene regulation and function. Isolation and functional characterization of a dioxininducible cyp1a regulatory region from zebrafish danio rerio zeruth, gary t. This book will be of keen interest not only to the typical readers of fish physiology but also to biomedical researchers, toxicologists and developmental biologists. Response of gene expression in zebrafish exposed to. Molecular docking of tcdd and leflunomide in zebrafish ahr isoforms. Dear colleagues, the zebrafish danio rerio has long been used as a model species for the toxicity testing of chemicals. Cyp1a, cyp1b1 and the cyp1cs are inducible by ahr agonists. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiologyand disease. Cytochrome p450 cyp enzymes are members of the hemoprotein superfamily, and are involved in the monooxygenation reactions of a wide range of endogenous and exogenous compounds in mammals and plants. The residues are displayed as sticks and colored by atom. Zeruth abstract cytochrome p4501a1 cyp1a1 is a phase i biotransformation enzyme involved in the metabolism of xenobiotics via the oxygenation of polycyclic aromatic hydrocarbons pahs including the carcinogen. The ideal aquarium for zebra fish contains clean oxygen rich water and has a lot of open area to swim in and some areas with dense vegetation.
Transgenic cyp1a reporter zebrafish reveals target tissues for tcdd. A guide for the laboratory use of zebrafish danio rerio. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Cyp enzymes catalyze oxidative transformation leading to activation or inactivation of many endogenous and exogenous chemicals, with consequences for normal physiology and disease processes. The retinal bipolar cells, otic vesicle, lateral line, pancreas, cloaca and pectoral fin bud are novel targets in zebrafish for tcdd. The little fish danio rerio holds secrets to understanding how vertebrates develop, how diseases like cancer work, and how we might one day learn how to regenerate human heart tissue. The zebrafish is a tropical fish native to southeast asia.
Using zebrafish in systems toxicology for developmental. In its larval stages it is transparent and as it matures to an adult it develops stripes that run along the length of the body and look blue in colour. Zebrafish antibodies primary antibodies sigmaaldrich. In this study, zebrafish eggs were exposed to a relatively low concentration 50 pgml of 2,3,7,8tetrachlorodibenzopdioxin tcdd for 72 h and then transferred to vehicletcddfree water for the remainder of the experiments. Youve just watched joves video on zebrafish development. The zebrafish has become one of the most important model organisms to study biologial processes in vivo. Its attractive slender body has numerous vertical, evenlyspaced thin orange stripes displaying an intricate pattern.
Guidance on the housing and care of zebrafish, danio rerio 3 acknowledgements the authors would like to thank the following people for their helpful comments during the preparation of this resource. This cuttingedge resource includes uptodate information on zebrafish physiology and the tools used to study it this book will be of keen interest not only to the typical readers of fish physiology but also to biomedical researchers, toxicologists an developmental biologists. A crystal structure of zebrafish cyp8a1 published in 2008 as part of a comparative structural analysis of prostacyclin synthases, shows that zebrafish and human cyp8a1 exhibit nearly identical ligandbound and ligandfree threedimensional structures, despite exhibiting only 45% identity. Fish cyp1a was the broadest and most active cyp for the substrates tested. To date no sex linked genes have been identified in zebrafish. Anderson home office, animals scientific procedures inspectorate, uk e. Identification and developmental expression of the full. Guidance on the housing and care ofzebrafishdanio rerio. Use features like bookmarks, note taking and highlighting while reading the convict cichlid. Mortality, heart rates, edema severity, cyp1a, and regucalcin gene expressions were investigated to study tcddinduced toxicity in zebrafish during the early life stage. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. The distinctive saltwater zebra fishes pterois, used in marine aquariums, have extremely large pectoral fins, numerous extremely poisonous spines, and colourful vertical stripes.
The glofish is a patented and trademarked brand of genetically engineered fluorescent fish. Furthermore, it is unknown when exactly zebrafish larvae attain adult cyp1a activity. You might struggle to imagine you have much in common with this small tropical freshwater fish, though you. Scientists develop zebrafish model for studying parkinson. Zebra cichlids, facts about mbuna pseudotropheus cichlids.
Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific. Shoal size and activity level are important parameters factoring shoaling decisionsin zebrafish. Zebra cichlids at has types of cichlids known as mbuna cichlids, fish information for pseudotropheus and rockdwelling malawi. Acknowledging materials and services please acknowledge the zebrafish international resource center in all publications resulting from the materials andor services we are providing to you.
Pritchard et al,2001 showed an individual preferred to be with the larger shoaland during tough compromising environmental conditions like lowertemperature, the choice shifted to the most active shoal. The cytochrome p450 2aa gene cluster in zebrafish danio. Please use this form to recommend updates to the information in zfin. Fish in general and zebra fish in particular present unique animal models for the in vivo study of highenergy. Zebra fish prefers neutral to slightly acidic water. Both zebrafish cyp1a and cyp1b1 metabolized er, br, and mr similar to mammalian cyp1a1 and cyp1b1, respectively. Both the head and body of the zebra dart goby are light green. The cytochrome p4501c cyp1c gene subfamily was recently discovered in fish, and zebrafish danio rerio cyp1c1 transcript has been cloned. Effects of copper oxide nanoparticles on developing. Transgenic cyp1a reporter zebrafish reveals target tissues for tcdd the retinal bipolar cells, otic vesicle, lateral line, pancreas, cloaca and pectoral fin bud are novel targets in zebrafish for tcdd.
Generation and application of a novel transgenic zebrafish line tg cyp1a. After fertilization, larvae survive with only the innate immune responses because adaptive immune system is morphologically and. The next few years will see the completion of large scale. Here we cloned the paralogous cyp1c2, showing that the amino acid sequence is 78% identical to cyp1c1, and examined gene structure and expression of cyp1a, cyp1b1, cyp1c1, and cyp1c2. The convict cichlid kindle edition by bradford risbert. Zebrafish types zebrafish aquaculture core facility at. No matter what youre looking for or where you are in the world, our global marketplace of sellers can help you find unique and affordable options. The reason for this drop in cyp1a activity remains unclear and it is also in contrast with a study of goldstone et al. Fritz large sample number, very quick and dirty, adequate for pcr this protocol is most suitable for samples consisting of 120, diploid, 23 day old embryos.
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